FLT3LG antibody (Biotin)

Catalog No. ABIN116011

This Rabbit Polyclonal antibody specifically detects FLT3LG in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for FLT3LG antibody (Biotin) (ABIN116011)

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3LG antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FLT3LG Antibody (Biotin)

Specificity: Recognizes Human Flt3-Ligand

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human Flt3-ligand.

Application Details

Application Notes: ELISADirect: To detect Human hFlt3-ligand by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human Flt3-ligand. Sandwich: To detect Human Flt3-ligand by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Flt3-ligand (PP1008P1 orPP1008P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human Flt3-ligand. Western Blot: To detect hFlt3-ligand by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for FLT3LG

Target: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Alternative Name: Flt3 Ligand

Background: Flt3 ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation. hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells.Synonyms: FLT3LG, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

Gene ID: 2323

NCBI Accession: NP_001450

UniProt: P49771

Pathways: RTK Signaling