CX3CL1 antibody (Biotin)

Catalog No. ABIN116015

The Rabbit Polyclonal anti-CX3CL1 antibody has been validated for WB and EIA. It is suitable to detect CX3CL1 in samples from Human.

Quick Overview for CX3CL1 antibody (Biotin) (ABIN116015)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CL1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CX3CL1 Antibody (Biotin)

Specificity: Human Fractalkine.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98%) recombinant hFractalkine (human Fractalkine)

Application Details

Application Notes: ELISA: (Direct): To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFractalkine. (Sandwich): To detect hFractalkine by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Fractalkine (PP1009P) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2, without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: Fractalkine / CX3CL1

Background: CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1

Gene ID: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane