CX3CL1 antibody

Catalog No. ABIN116017

This Rabbit Polyclonal antibody specifically detects CX3CL1 in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CX3CL1 antibody (ABIN116017)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CX3CL1 Antibody

Specificity: This antibody is specific to Human Fractalkine.

Purification: Affinity Chromatography

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human Fractalkine (Cat.-No PA057). Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: Neutralization. Sandwich ELISA: To detect Human Fractalkine by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine(PP1009B1 or PP1009B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Fractalkine. Western Blot: To detect Human Fractalkine by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Alternative Name: Fractalkine / CX3CL1

Background: CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1

Gene ID: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane