IL-8 antibody

Catalog No. ABIN116091

This anti-IL-8 antibody is a Rabbit Polyclonal antibody detecting IL-8 in WB, IHC (p), IF, EIA and Func. Suitable for Human.

Quick Overview for IL-8 antibody (ABIN116091)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-8 Antibody

Purification: Affinity Chromatography employing immobilized hIL-8 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human Interleukin 8.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections1.0 μg/mL for two hours at room temperature or 0.25 μg/mL overnight at 4 °C. An HRPlabeled polymer detection system was used with a non-alcohol soluble AEC chromogenand a proteinase K antigen retrieval.
Optimal concentrations and conditions may vary. NeutralizationTo yield one-half maximal inhibition (ND50) of the biological activity of human IL-8 (100 ng/mL), a concentration of 2-3 μg/mL of this antibody is required. ELISAIndirect: To detect hIL-8 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hIL-8. Sandwich: To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8

Background: IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

Gene ID: 9606

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy