CCL8 antibody

Catalog No. ABIN116143

This Rabbit Polyclonal antibody specifically detects CCL8 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL8 antibody (ABIN116143)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL8 Antibody

Specificity: Human Macrophage Chemotactic Protein-2 (MCP-2).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMCP-2.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-2 (100.00 ng/mL), a concentration of 12-16 μg/mL of this antibody is required. ELISA: To detect hMCP-2 by Indirect ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5-0.2 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMCP-2. Sandwich: To detect hMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-2 (PP1045B1/B2) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening!

Storage: 4 °C

Storage Comment: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: MCP2 / CCL8

Background: MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8

Gene ID: 6355

NCBI Accession: NP_005614

UniProt: P80075