CCL20 antibody

Catalog No. ABIN116171

The Rabbit Polyclonal anti-CCL20 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL20 in samples from Human.

Quick Overview for CCL20 antibody (ABIN116171)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL20 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL20 Antibody

Specificity: Human MIP-3 alpha (EXODUS-1, LARC).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant human Macrophage Inflammatory Protein 3 alpha (hMIP-3 alpha).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman MIP-3 alpha (100 ng/mL), a concentration of 15.0 - 18.0g/mL of this antibody isrequired. ELISA: To detect MIP-3 alpha by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antibody allows thedetection of 0.2 - 0.4 ng/well of recombinant MIP-3 alpha. Western Blot: This antibody can be used at a concentration of 0.1 - 0.2 μg/mL The detectionlimit for recombinant MIP-3 alpha is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry on frozen (use 1 μg/mL) and paraffin embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C for one year. Following reconstitution the antibody can be stored at 2-8 °C for 2 weeks.

Expiry Date: 12 months

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP3 alpha / CCL20

Background: Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. It binds to CCR6.Synonyms: Beta chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, CCL20, DC marker, Dendritic Cell Marker, Exodus-1, LARC, Liver and activation-regulated chemokine, MIP-3 alpha, MIP3A, Macrophage inflammatory protein 3 alpha, SCYA20, Small-inducible cytokine A20

Gene ID: 6364

NCBI Accession: NP_001123518

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection