CCL18 antibody (Biotin)

Catalog No. ABIN116177

The Rabbit Polyclonal anti-CCL18 antibody has been validated for WB and EIA. It is suitable to detect CCL18 in samples from Human.

Quick Overview for CCL18 antibody (Biotin) (ABIN116177)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL18 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL18 Antibody (Biotin)

Specificity: Specific for human Macrophage Inflammatory Protein-4 (hMIP-4) (PARC).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMIP-4.

Application Details

Application Notes: ELISA: To detect hMIP-4 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMIP-4Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL18

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: MIP4 / CCL18

Background: This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.Synonyms: AMAC1, Alternative macrophage activation-associated CC chemokine 1, C-C motif chemokine 18, CC chemokine PARC, DCCK1, Dendritic cell chemokine 1, MIP-4, Macrophage inflammatory protein 4, PARC, Pulmonary and activation-regulated chemokine, SCYA18, Small-inducible cytokine A18

Gene ID: 6362

NCBI Accession: NP_002979

UniProt: P55774