CCL18 antibody

Catalog No. ABIN116179

This Rabbit Polyclonal antibody specifically detects CCL18 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL18 antibody (ABIN116179)

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL18 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL18 Antibody

Specificity: Specific for Human Macrophage Inflammatory Protein-4 (MIP-4).

Purification: Affinity Chromatography using an immobilized Human MIP-4 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human MIP-4

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-4 (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required. ELISA: (Indirect): To detect hMIP-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hMIP-4. (Sandwich): To detect hMIP-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MIP-4 (PP1054B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4. Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL18

Target: CCL18 (Chemokine (C-C Motif) Ligand 18 (Pulmonary and Activation-Regulated) (CCL18))

Alternative Name: MIP4 / CCL18

Background: This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.Synonyms: AMAC1, Alternative macrophage activation-associated CC chemokine 1, C-C motif chemokine 18, CC chemokine PARC, DCCK1, Dendritic cell chemokine 1, MIP-4, Macrophage inflammatory protein 4, PARC, Pulmonary and activation-regulated chemokine, SCYA18, Small-inducible cytokine A18

Gene ID: 6362

NCBI Accession: NP_002979

UniProt: P55774