CXCL9 antibody (Biotin)

Catalog No. ABIN116181

The Biotin-conjugated Rabbit Polyclonal anti-CXCL9 antibody (ABIN116181) specifically detects CXCL9 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for CXCL9 antibody (Biotin) (ABIN116181)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL9 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL9 Antibody (Biotin)

Specificity: Detects Human Monokine induced by interferon-gamma (MIG).

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived Human recombinant MIG

Application Details

Application Notes: ELISA: To detect hMIG by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMIG. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIG is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: CXCL9

Background: MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.Synonyms: C-X-C motif chemokine 9, CMK, CXCL9, Gamma-interferon-induced monokine, MIG, SCYB9, Small-inducible cytokine B9

Gene ID: 4283

NCBI Accession: NP_002407

UniProt: Q07325