Western Blot (0.1-0.2 μg/mL will detect 1.5-3.0 ng/lane under reducing or non-reducingconditions). ELISA (0.5 μg/mL (100 l/well) will detect 0.2-0.4 ng/well of MIG)Neutralisation (5 - 7 μg/mL will neutralize 50 % of the biological activity of 100 ng/mL ofMIG. Immunohistochemistry on frozen sections or cells has been described: 1 μg/mLrecommended for starting dilution. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage
4 °C/-20 °C
Storage Comment
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
CXCL9
(gamma-Interferon-Induced Monokine (CXCL9))
Alternative Name
CXCL9
Background
MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.Synonyms: C-X-C motif chemokine 9, CMK, CXCL9, Gamma-interferon-induced monokine, MIG, SCYB9, Small-inducible cytokine B9