Nerve Growth Factor antibody (Biotin)

Catalog No. ABIN116189

The Rabbit Polyclonal anti-Nerve Growth Factor antibody has been validated for WB and EIA. It is suitable to detect Nerve Growth Factor in samples from Human.

Quick Overview for Nerve Growth Factor antibody (Biotin) (ABIN116189)

Target: Nerve Growth Factor (NGF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Nerve Growth Factor Antibody (Biotin)

Specificity: Specific for Human beta NGF (Nerve Growth Factor). Other species not tested.

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hbeta-NGF (Anti-human beta-Nerve Growth Factor).

Application Details

Application Notes: ELISA: To detect hbeta-NGF by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthbeta-NGF. Western Blot: To detect hbeta-NGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hbeta-NGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gene ID: 4803

NCBI Accession: NP_002497

UniProt: P01138

Pathways: Regulation of Cell Size