PF4 antibody (Biotin)

Catalog No. ABIN116213

The Rabbit Polyclonal anti-PF4 antibody has been validated for WB and EIA. It is suitable to detect PF4 in samples from Human.

Quick Overview for PF4 antibody (Biotin) (ABIN116213)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PF4 Antibody (Biotin)

Specificity: Human Platelet Factor 4 (hPF-4).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hPF-4 (human PF- 4).

Application Details

Application Notes: ELISA: To detect hPF-4 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or in aliquots at -20 °C for longer.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4 / PF4

Background: Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gene ID: 5196

NCBI Accession: NP_002610

UniProt: P02776