PF4 antibody

Catalog No. ABIN116215

This Rabbit Polyclonal antibody specifically detects PF4 in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for PF4 antibody (ABIN116215)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-PF4 Antibody

Specificity: This antibody reacts with Human Platelet Factor 4 (PF-4).

Purification: Affinity Chromatography employing immobilized Human PF-4 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhPF?4 (50 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hPF?4 by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hPF?4. Western Blot: To detect hPF?4 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPF?4 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening.Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4 / PF4

Background: Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4

Gene ID: 5196

NCBI Accession: NP_002610

UniProt: P02776