CXCL12 antibody (Biotin)

Catalog No. ABIN116227

This Rabbit Polyclonal antibody specifically detects CXCL12 in WB and EIA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for CXCL12 antibody (Biotin) (ABIN116227)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL12 Antibody (Biotin)

Specificity: Recognizes Stromal Cell Derived Factor-1 alpha (SDF-1 alpha).

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha.

Application Details

Application Notes: ELISA: To detect hSDF-1alpha by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hSDF-1alpha. To detect hSDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a polyclonal Anti-Human SDF-1alpha (PP1067P) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1alpha. Western Blot: To detect hSDF-1alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSDF-1alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-CXCL12 antibody (Biotin) (ABIN116227)

Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12 / SDF1

Background: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Gene ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling