CXCL12 antibody

Catalog No. ABIN116229

This anti-CXCL12 antibody is a Rabbit Polyclonal antibody detecting CXCL12 in WB, IHC (p), EIA and Func. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for CXCL12 antibody (ABIN116229)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL12 Antibody

Specificity: This antibody detects Human Stromal Cell Derived Factor-1 alpha. Other species not tested.

Purification: Affinity Chromatography employing immobilized Human SDF1a matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α (100 ng/mL),a concentration of 2-4 μg/mL of this antibody is required. ELISA: Indirect: Using 100 μL/well antibody solution, a concentration of 0.5-2.0 μg/mL is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hSDF-1α. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.5 - 2.0 μg/mL isrequired. In conjunction with Biotinylated Anti-Human SDF-1α (PP1067B) as a detectionantibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hSDF-1α. Western Blot: 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hSDF-1α is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should protected from light.

References for anti-CXCL12 antibody (ABIN116229)

Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12 / SDF1

Background: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Gene ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling