CXCL12 antibody

Catalog No. ABIN116233

This Goat Polyclonal antibody specifically detects CXCL12 in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CXCL12 antibody (ABIN116233)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL12 Antibody

Specificity: Reacts with Stromal Cell Derived Factor-1 beta (SDF-1 beta).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: E. Coli derived Recombinant recombinant Human SDF-1 beta

Isotype: IgG

Application Details

Application Notes: Indirect ELISA: To detect Human SDF-1 beta by Indirect ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with compatible secondary reagents, allows thedetection of 0.2-0.4 ng/well of recombinant Human SDF-1 beta. Sandwich ELISA: To detect Human SDF-1 beta by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SDF-1 beta(PP1068B1 or PP1068B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1 beta. Western Blot: To detect Human SDF-1 beta by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSDF-1 bet is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening! Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12 / SDF1

Background: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Molecular Weight: approx. 150 kDa

Gene ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling