CXCL10 antibody (Biotin)

Catalog No. ABIN116269

This Rabbit Polyclonal antibody specifically detects CXCL10 in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for CXCL10 antibody (Biotin) (ABIN116269)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL10 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL10 Antibody (Biotin)

Specificity: Reacts with Interferon-gamma Inducible Protein-10.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse IP-10.

Application Details

Application Notes: Direct ELISA: To detect Mouse IP-10 by Direct ELISA (using 100 ml/well antibody solution)this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylated antibodyallows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Sandwich ELISA: To detect Mouse IP-10 by Sandwich ELISA (using 100 ml/well antibodysolution), a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedantibody, in conjuction with anti Mouse IP10 antibody (cat. PP1083P) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IP-10. Western Blot: To detect Mouse IP-10 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Mouse IP-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Alternative Name: CXCL10 / IP-10

Background: Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.Synonyms: 10 kDa interferon-gamma-induced protein, C-X-C motif chemokine 10, Gamma-IP10, INP10, SCYB10, Small-inducible cytokine B10

Gene ID: 15945

NCBI Accession: NP_067249

UniProt: P17515