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RANKL antibody

TNFSF11 Reactivity: Human WB, IHC (p), EIA, Func Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN116282
  • Target See all RANKL (TNFSF11) Antibodies
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Reactivity
    • 167
    • 130
    • 113
    • 46
    • 2
    • 2
    Human
    Host
    • 156
    • 31
    • 16
    • 5
    • 1
    Rabbit
    Clonality
    • 171
    • 36
    • 2
    Polyclonal
    Conjugate
    • 77
    • 18
    • 17
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    This RANKL antibody is un-conjugated
    Application
    • 170
    • 78
    • 78
    • 64
    • 38
    • 30
    • 29
    • 20
    • 13
    • 12
    • 7
    • 7
    • 5
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    Specificity
    This antibody reacts with soluble RANK Ligand.
    Purification
    Affinity Chromatography.
    Immunogen
    Highly pure (>98%) recombinant Human sRANKL.
    Top Product
    Discover our top product TNFSF11 Primary Antibody
  • Application Notes
    Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 μg/mL withan overnight incubation at 4 °C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2 without preservatives
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Target
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Alternative Name
    CD254 / RANKL (TNFSF11 Products)
    Synonyms
    CD254 antibody, ODF antibody, OPGL antibody, OPTB2 antibody, RANKL antibody, TRANCE antibody, hRANKL2 antibody, sOdf antibody, TNFSF11 antibody, Ly109l antibody, OPG antibody, Trance antibody, cd254 antibody, odf antibody, opgl antibody, optb2 antibody, rankl antibody, sodf antibody, tnfsf11 antibody, trance antibody, TNF superfamily member 11 antibody, tumor necrosis factor (ligand) superfamily, member 11 antibody, TNF receptor superfamily member 11b antibody, TNF superfamily member 11 L homeolog antibody, TNFSF11 antibody, Tnfsf11 antibody, TNFRSF11B antibody, tnfsf11.L antibody
    Background
    RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
    Gene ID
    8600
    NCBI Accession
    NP_003692
    UniProt
    O14788
    Pathways
    NF-kappaB Signaling
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