CCL15 antibody

Catalog No. ABIN116285

The Goat Polyclonal anti-CCL15 antibody has been validated for WB, EIA and Func. It is suitable to detect CCL15 in samples from Human.

Quick Overview for CCL15 antibody (ABIN116285)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL15 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL15 Antibody

Specificity: Macrophage Inflammatory Protein-5 (MIP-5).

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMIP-5.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-5 (100.00 ng/mL), a concentration of 2.0-3.0 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: MIP5 / CCL15

Background: Macrophage inflammatory protein 5 (CCL15) is a member of the human beta-chemokine family and is chemotactic for T cells and monocytes but not neutrophils, eosinophils, or B cells. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor but also binds to CCR3.Synonyms: C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, MIP-5, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, NCC3, SCYA15, Small-inducible cytokine A15

Gene ID: 6359

NCBI Accession: NP_116741

UniProt: Q16663