CCL16 antibody (Biotin)

Catalog No. ABIN116291

The Goat Polyclonal anti-CCL16 antibody has been validated for WB and EIA. It is suitable to detect CCL16 in samples from Human.

Quick Overview for CCL16 antibody (Biotin) (ABIN116291)

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL16 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL16 Antibody (Biotin)

Specificity: Human LEC

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hLEC.

Application Details

Application Notes: ELISA: To detect hLEC by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLEC. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLEC is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody diluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CCL16

Target: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Alternative Name: NCC4 / CCL16

Background: LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.Synonyms: C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, ILINCK, LCC-1, LCC1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, SCYA16

Gene ID: 6360

NCBI Accession: NP_004581

UniProt: O15467