CXCL11 antibody

Catalog No. ABIN116297

The Rabbit Polyclonal anti-CXCL11 antibody has been validated for WB, EIA and Func. It is suitable to detect CXCL11 in samples from Human.

Quick Overview for CXCL11 antibody (ABIN116297)

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL11 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CXCL11 Antibody

Specificity: This antibody reacts with Human I-TAC

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hI-TAC.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [NND50] of the biological activity ofhI-TAC (100 ng/mL), a concentration of 7.0-8.0 μg/mL of this antibody is required. ELISA: To detect hI-TAC by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant hI-TAC. Western Blot: To detect hI-TAC by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hI-TAC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: CXCL11 / ITAC

Background: This member of the the intercrine alpha (chemokine CxC) family is chemotactive for interleukin-activated T cells but not unstimulated T cells, neutrophils or monocytes. It induces calcium release in activated T cells and binds to CXCR3. It may play an important role in CNS diseases which involve T cell recruitment and in skin immune responses. It is expressed at high levels in peripheral blood leukocytes, pancreas and liver astrocytes and at moderate levels in thymus, spleen and lung. It is induced by interferon gamma and interferon beta.Synonyms: Beta-R1, C-X-C motif chemokine 11, H174, I-TAC, IP-9, Interferon-gamma-inducible protein 9, Interferon-inducible T-cell alpha chemoattractant, SCYB11, SCYB9B, Small-inducible cytokine B11

Gene ID: 6373

NCBI Accession: NP_005400

UniProt: O14625