alpha Defensin 1 antibody (Biotin)

Catalog No. ABIN116327

The Goat Polyclonal anti-alpha Defensin 1 antibody has been validated for EIA. It is suitable to detect alpha Defensin 1 in samples from Human.

Quick Overview for alpha Defensin 1 antibody (Biotin) (ABIN116327)

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Defensin 1 antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Product Details anti-alpha Defensin 1 Antibody (Biotin)

Specificity: Defensin alpha 1-3 (NP-1)

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived Human recombinant NP-1.

Application Details

Application Notes: ELISA: To detect hNP-1 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNP-1 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C or in aliquots at -20 °C long term.

Target Details for alpha Defensin 1

Target: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Alternative Name: Defensin alpha 1

Background: Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30 % of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3

Gene ID: 1667

NCBI Accession: NP_001035965

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin