CCL25 antibody

Catalog No. ABIN116335

This Rabbit Polyclonal antibody specifically detects CCL25 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL25 antibody (ABIN116335)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL25 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL25 Antibody

Specificity: Thymus Expressed Chemokine (TECK)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTECK (100 ng/mL), a concentration of 10.0- 15.0 μg/mL of this antibody is required. ELISA: To detect hTECK by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK / CCL25

Background: Synonyms: C-C motif chemokine 25, Chemokine TECK, SCYA25, Small-inducible cytokine A25, Thymus-expressed chemokine

Gene ID: 6370

NCBI Accession: NP_005615

UniProt: O15444