IL-10 antibody

Catalog No. ABIN116339

The Rabbit Polyclonal anti-IL-10 antibody has been validated for WB, EIA and Func. It is suitable to detect IL-10 in samples from Rat.

Quick Overview for IL-10 antibody (ABIN116339)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-10 Antibody

Specificity: Recognizes Rat IL10. Other species not tested.

Purification: Affinity Chromatography employing immobilized Rat IL-10 matrix.

Immunogen: Highly pure (>98%) recombinant Rat IL-10.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-10 (30.00 ng/mL), a concentration of 0.45-0.80 μg/mL of this antibody is required. ELISAIndirect: To detect Rat IL-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat IL-10. Sandwich: To detect Rat IL-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat IL-10 (PP1115B1 or PP1115B2) as a

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Interleukin-10 / IL10

Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73 % homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF

Gene ID: 25325

NCBI Accession: NP_036986

UniProt: P29456

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints