FGF17 antibody (Biotin)

Catalog No. ABIN116373

The Biotin-conjugated Goat Polyclonal anti-FGF17 antibody (ABIN116373) specifically detects FGF17 in EIA. The antibody is reactive with Human samples.

Quick Overview for FGF17 antibody (Biotin) (ABIN116373)

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF17 antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Product Details anti-FGF17 Antibody (Biotin)

Specificity: This antibody reacts with FGF-17

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hFGF-17.

Application Details

Application Notes: ELISA: (Direct): To detect hFGF-17 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-17. (Sandwich): To detect hFGF-17 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human FGF-17 (PP1130P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-17. Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-17 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for FGF17

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Alternative Name: FGF17

Background: FGF17 belongs to the heparin-binding growth factors family. It may be a signaling molecule in the induction and patterning of the embryonic brain and is preferentially expressed in the embryonic brain. There are two named isoforms.Synonyms: FGF-17, Fibroblast growth factor 17

Gene ID: 8822

NCBI Accession: NP_003858

UniProt: O60258

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway