FGF17 antibody

Catalog No. ABIN116375

The Goat Polyclonal anti-FGF17 antibody is suitable to detect FGF17 in samples from Human. It has been validated for WB, EIA and Func.

Quick Overview for FGF17 antibody (ABIN116375)

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF17 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-FGF17 Antibody

Specificity: This antibody recognizes FGF-17

Purification: Affinity Chromatography employing immobilized Human FGF-17 matrix.

Immunogen: Highly pure (>98%) recombinant Human FGF-17

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman FGF-17 (200.0 ng/mL), a concentration of 2.1-3.2 μg/mL of this antibody is required. Sandwich ELISA: To detect Human FGF-17 by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Human FGF-17 (PP1130B1 orPP1130B2) as Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinantHuman FGF-17. Western Blot: To detect Human FGF-17 by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human FGF-17 is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile Water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for FGF17

Target: FGF17 (Fibroblast Growth Factor 17 (FGF17))

Alternative Name: FGF17

Background: FGF17 belongs to the heparin-binding growth factors family. It may be a signaling molecule in the induction and patterning of the embryonic brain and is preferentially expressed in the embryonic brain. There are two named isoforms.Synonyms: FGF-17, Fibroblast growth factor 17

Gene ID: 8822

NCBI Accession: NP_003858

UniProt: O60258

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway