CXCL16 antibody (Biotin)

Catalog No. ABIN116403

This anti-CXCL16 antibody is a Rabbit Polyclonal antibody detecting CXCL16 in WB and EIA. Suitable for Human.

Quick Overview for CXCL16 antibody (Biotin) (ABIN116403)

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL16 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL16 Antibody (Biotin)

Specificity: This antibody recognizes Human CXCL16. Other species not tested.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hCXCL16.

Application Details

Application Notes: Direct ELISA: To detect hCXCL16 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1 μg/mL of this antibody is required. This Biotinylated antibody, allowsthe detection of 0.2-0.4 ng/well of recombinant hCXCL16. Sandwich ELISA: To detect hCXCL16 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylatedantibody, in conjunction with Affinity purified anti-Human CXCL16 antibody (PP1204P1 orPP1204P2) as a Capture antibody, allows the detection of 0.2-0.4 ng/well of recombinanthCXCL16. Western Blot: To detect hCXCL16 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. The detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS solution containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL16

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Alternative Name: CXCL16

Background: CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.Synonyms: C-X-C motif chemokine 16, SCYB16, SR-PSOX, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16

Gene ID: 58191

NCBI Accession: NP_001094282

UniProt: Q9H2A7