CXCL16 antibody

Catalog No. ABIN116405

The Rabbit Polyclonal anti-CXCL16 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect CXCL16 in samples from Human. There is 1 publication available.

Quick Overview for CXCL16 antibody (ABIN116405)

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-CXCL16 Antibody

Specificity: This antibody recognizes Human CXCL16. Other species not tested.

Purification: Affinity Chromatography employing immobilized hCXCL16 matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human CXCL16

Application Details

Application Notes: Indirect ELISA: To detect hCXCL16 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of 0.2-0.4 ng/well ofrecombinant hCXCL16. Sandwich ELISA: To detect hCXCL16 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, inconjunction with a biotinylated anti-Human CXCL16 (PP1204B1 or PP1204B2) as a detectionantibody, allows the detection of 0.2-0.4 ng/well of recombinant hCXCL16. Western Blot: To detect hCXCL16 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. The detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: The antibody stained FFPE Sections of human colorectaladenocarcinoma tissue. An HRP-labelled polymer detection system was used with a DAB chromogen). Antigenretrieval is not required for these conditions. Working Dilution: 0.5 μg/mL, overnight incubation at 4 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-CXCL16 antibody (ABIN116405)

Gutwein, Abdel-Bakky, Schramme, Doberstein, Kämpfer-Kolb, Amann, Hauser, Obermüller, Bartel, Abdel-Aziz, El Sayed, Pfeilschifter: "CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2061-72, (2009) (PubMed).

Target Details for CXCL16

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Alternative Name: CXCL16

Background: CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.Synonyms: C-X-C motif chemokine 16, SCYB16, SR-PSOX, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16

Gene ID: 58191

NCBI Accession: NP_001094282

UniProt: Q9H2A7