IL18BP antibody

Catalog No. ABIN116421

This anti-IL18BP antibody is a Goat Polyclonal antibody detecting IL18BP in WB and EIA. Suitable for Mouse.

Quick Overview for IL18BP antibody (ABIN116421)

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL18BP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL18BP Antibody

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-18P.

Application Details

Application Notes: Neutralization. ELISA: To detect mIL-18BP by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant mIL-18BP. Western Blot: To detect mIL-18BP by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-18BP is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5 X PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL18BP

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Alternative Name: IL18BP

Background: IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.Synonyms: IL-18BP, Interleukin-18-binding protein, Tadekinig-alfa

Gene ID: 16068

NCBI Accession: NP_034661

UniProt: Q9Z0M9