CXCL12 antibody (Biotin)

Catalog No. ABIN116455

This Goat Polyclonal antibody specifically detects CXCL12 in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for CXCL12 antibody (Biotin) (ABIN116455)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL12 Antibody (Biotin)

Specificity: Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Mouse SDF-1 alpha. Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: Direct ELISA: To detect Mouse SDF-1 alpha by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.15-0.30 μg/mL. Used inconjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Mouse SDF-1 alpha. Sandwich ELISA: To detect Mouse SDF-1 alpha by sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. Thisbiotinylated polyclonal antibody, in conjunction with Anti-Mouse SDF-1 alpha (PP1217P1 orPP1217P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant MOuse SDF-1 alpha. Western Blot: To detect Mouse SDF-1alpha by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Mouse SDF-1 alpha is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: CXCL12 / SDF1

Background: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Molecular Weight: approx. 150 kDa

Gene ID: 20315

NCBI Accession: NP_001012495

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling