BMP2 antibody

Catalog No. ABIN116465

The Rabbit Polyclonal anti-BMP2 antibody has been validated for WB, EIA and Func. It is suitable to detect BMP2 in samples from Human.

Quick Overview for BMP2 antibody (ABIN116465)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-BMP2 Antibody

Specificity: The antibody recognizes Human BMP2. Other species not tested.

Purification: Affinity Chromatography employing immobilized Human BMP-2 matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human BMP-2.

Application Details

Application Notes: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200 ng/mL), a concentration of 1.3-1.9 μg/mL of this antibody is required. ELISAIndirect: To detect hBMP-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBMP-2. Sandwich: To detect hBMP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human BMP-2 (PP1219B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2. Western BlotTo detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2 / BMP2A

Background: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.Synonyms: BMP-2, BMP-2A, Bone morphogenetic protein 2

Gene ID: 650

NCBI Accession: NP_001191

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation