IL-22 antibody (Biotin)

Catalog No. ABIN116483

The Rabbit Polyclonal anti-IL-22 antibody has been validated for WB and EIA. It is suitable to detect IL-22 in samples from Human.

Quick Overview for IL-22 antibody (Biotin) (ABIN116483)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-22 Antibody (Biotin)

Specificity: Recognizes Human Interleukin-22 (IL-22).

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human IL-22 (hIL-22).

Application Details

Application Notes: ELISADirect: To detect Human IL-22 by direct ELISA (using 100 μL/well) a concentration of approx. 1.0 μg/mL of this antibody is required. The Biotinylated antibody allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Sandwich: To detect Human IL-22 by Sandwich ELISA (using 100 μL/well) a concentration of0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody, in conjuction withanti-Human IL22 (PP1224P1 or PP1224P2) as a capture antibody, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human IL-22. Western blot: To detect Human IL-22 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-22 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with sterile PBS solution containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: Interleukin-22 / IL22

Background: IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF

Gene ID: 50616

NCBI Accession: NP_065386

UniProt: Q9GZX6