IL-22 antibody

Catalog No. ABIN116493

This anti-IL-22 antibody is a Rabbit Polyclonal antibody detecting IL-22 in WB and EIA. Suitable for Mouse.

Quick Overview for IL-22 antibody (ABIN116493)

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-22 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-22 Antibody

Specificity: This antibody recognizes murine IL-22.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-22 (murine Interleukin-22).

Application Details

Application Notes: ELISAIndirect: To detect mIL-22 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant mIL-22. Sandwich: To detect mIL-22 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-22 (PP1226B1 or PP1226B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-22. Western blotTo detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: PBS, pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: Interleukin-22 / IL22

Background: IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production, this may have therapeutic potential, particularly in the treatment of asthma.Synonyms: Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF

Gene ID: 50929

NCBI Accession: NP_058667

UniProt: Q9JJY9