RFX5 antibody (AA 320-494)

Catalog No. ABIN116532

This anti-RFX5 antibody is a Rabbit Polyclonal antibody detecting RFX5 in WB, IP, EIA and EMSA. Suitable for Human and Mouse.

Quick Overview for RFX5 antibody (AA 320-494) (ABIN116532)

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Product Details anti-RFX5 Antibody

Binding Specificity: AA 320-494

Specificity: Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa. Human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is of the region between amino acids 320 to 494. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

Purification: Prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed byimmunoblot and found to be reactive against RFX5 (aa 320 to494) from a variety of fibroblast and B-cell lysates at a dilution of 1: 1,000 followed byreaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive againstRFX5 complexes using 0.5 to 1.0 l per assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.68 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: RFX5

Background: RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5

Gene ID: 5993

NCBI Accession: NP_000440

UniProt: P48382