Clusterin antibody

Catalog No. ABIN116548

This Goat Polyclonal antibody specifically detects Clusterin in WB, IP, IHC (p), EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Clusterin antibody (ABIN116548)

Target: Clusterin (CLU)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Clusterin Antibody

Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Purification: Immunoaffinity chromatography

Immunogen: ApoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Application Details

Application Notes: Has been used for indirect trapping ELISA for quantitation of antigen in serum using astandard curve, for immunoprecipitation and for western blotting for highly sensitivequalitative analysis. Recommended dilutions: ELISA 1: 5,000 - 1: 10,000. Western blot 1: 5,000 - 1: 10,000.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: This product is stable at 2-8 °C as an undiluted liquid for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 °C or below.

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Apolipoprotein J (Apo J)

Background: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gene ID: 1191

NCBI Accession: NP_976084

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling