ADH1 antibody

Catalog No. ABIN116588

The Rabbit Polyclonal anti-ADH1 antibody has been validated for WB and EIA. It is suitable to detect ADH1 in samples from Saccharomyces cerevisiae.

Quick Overview for ADH1 antibody (ABIN116588)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ADH1 Antibody

Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.

Purification: Delipidation and defibrination.

Immunogen: Full length Alcohol Dehydrogenase isolated from yeast.

Application Details

Application Notes: ELISA: 1/5,000-1/25,000). Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding tomonomeric alcohol dehdrogenase in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: Alcohol Dehydrogenase 1

Background: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C

Gene ID: 854068

NCBI Accession: NP_014555

UniProt: P00330