Alkaline Phosphatase antibody

Catalog No. ABIN116596

This Rabbit Polyclonal antibody specifically detects Alkaline Phosphatase in WB, IP and EIA. It exhibits reactivity toward E. coli.

Quick Overview for Alkaline Phosphatase antibody (ABIN116596)

Target: Alkaline Phosphatase (ALP)

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Alkaline Phosphatase Antibody

Specificity: This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

Purification: Delipidation and Defibrination

Immunogen: Alkaline Phosphatase [E.coli].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 75.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Target Details for Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background: Synonyms: JW0374, b0383, phoA

Gene ID: 945041

NCBI Accession: AP_001034

UniProt: P00634