CA2 antibody

Catalog No. ABIN116657

The Rabbit Polyclonal anti-CA2 antibody is suitable to detect CA2 in samples from Human. It has been validated for WB and EIA.

Quick Overview for CA2 antibody (ABIN116657)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CA2 Antibody

Specificity: This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [human erythrocytes].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination

Immunogen: Carbonic Anhydrase II from Human Erythrocytes. Genename: CA2

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA (1/115,000) and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of CarbonicAnhydrase II [Human Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) R1364HRP and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 5,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE! This antibody should be protected from light as the PE-labelled product is photosensitive.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase 2

Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gene ID: 760

NCBI Accession: NP_000058

UniProt: P00918