Choline Oxidase antibody

Catalog No. ABIN116681

The Goat Polyclonal anti-Choline Oxidase antibody is suitable to detect Choline Oxidase in samples from Alcaligenes. It has been validated for WB and EIA.

Quick Overview for Choline Oxidase antibody (ABIN116681)

Target: Choline Oxidase

Reactivity: Alcaligenes

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Choline Oxidase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Choline Oxidase [Alcaligenes species].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Choline Oxidase[Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Choline Oxidase

Target: Choline Oxidase

Background: Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.

Gene ID: 512

UniProt: P16101