DNASE1 antibody

Catalog No. ABIN116689

This Rabbit Polyclonal antibody specifically detects DNASE1 in WB and EIA. It exhibits reactivity toward Cow.

Quick Overview for DNASE1 antibody (ABIN116689)

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNASE1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DNASE1 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Deoxyribonuclease I from bovine pancreas.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofDeoxyribonuclease I [Bovine Pancreas] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] code R1364HRP and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 100 to 1: 1,000 of the reconstitution concentration is suggested forthis product.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details for DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: Deoxyribonuclease-1

Background: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.Synonyms: DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I

Gene ID: 1773

NCBI Accession: NP_005214

UniProt: P24855