Glucose-6-Phosphate Dehydrogenase antibody

Catalog No. ABIN116713

This Goat Polyclonal antibody specifically detects Glucose-6-Phosphate Dehydrogenase in WB and EIA. It exhibits reactivity toward Bacillus.

Quick Overview for Glucose-6-Phosphate Dehydrogenase antibody (ABIN116713)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glucose Dehydrogenase [Bacillus].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of GlucoseDehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Goat IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,500 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. For extended storage, aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose Dehydrogenase

Background: Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.Synonyms: BSU03930, Glucose 1-dehydrogenase, gdh

Gene ID: 938261

NCBI Accession: NP_388275

UniProt: P12310

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones