Luciferase antibody

Catalog No. ABIN116764

This Goat Polyclonal antibody specifically detects Luciferase in WB, IP, IHC (fro) and EIA. It exhibits reactivity toward Photinus pyralis.

Quick Overview for Luciferase antibody (ABIN116764)

Target: Luciferase

Reactivity: Photinus pyralis

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-Luciferase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Luciferase [Firefly].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Immunhistochemistry and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Luciferase[Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 20000 to1: 100000 of the reconstitution concentration is suggested for this product. For Western Blot, a recommended working dilution is 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 95.0 mg/mL (by Refractometry)

Buffer: PBS, pH 7.2 containing 0.01 % sodium azides as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Luciferase

Target: Luciferase

Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase

Gene ID: 7054

UniProt: P08659