PRSS1 antibody

Catalog No. ABIN116829

This anti-PRSS1 antibody is a Rabbit Polyclonal antibody detecting PRSS1 in WB and EIA. Suitable for Cow.

Quick Overview for PRSS1 antibody (ABIN116829)

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRSS1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRSS1 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Trypsin [Bovine Pancreas].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 g of Trypsin [Bovine Pancreas] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to 1: 4,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.

Target Details for PRSS1

Target: PRSS1 (Protease, serine, 1 (Trypsin 1) (PRSS1))

Alternative Name: Trypsin-1 / PRSS1

Background: Synonyms: Beta-trypsin, Cationic trypsinogen, Serine protease 1, TRP1, TRY1, TRYP1, Trypsin I

Gene ID: 615026

NCBI Accession: NP_001107199

UniProt: P00760