LAG3 antibody (N-Term) (Atto 647N)

Catalog No. ABIN1169111

The Mouse Monoclonal anti-LAG3 antibody has been validated for ICC and FACS. It is suitable to detect LAG3 in samples from Human.

Quick Overview for LAG3 antibody (N-Term) (Atto 647N) (ABIN1169111)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is conjugated to Atto 647N

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 17B4

Product Details anti-LAG3 Antibody (Atto 647N)

Binding Specificity: N-Term

Specificity: Recognizes human LAG-3.

Cross-Reactivity: Human

Purity: >95 % (SDS-PAGE)

Immunogen: Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (lambdaabs (max): 645nm, lambdaem (max): 669nm, epsilonmax: 120'000).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.

Expiry Date: 12 months

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG-3

Background: Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints