Caspase 4/11 p20 (AA 81-373) antibody (Biotin)

Catalog No. ABIN1169420

The Mouse Monoclonal anti-Caspase 4/11 p20 antibody is suitable to detect Caspase 4/11 p20 in samples from Human and Mouse. It has been validated for IP and WB.

Quick Overview for Caspase 4/11 p20 (AA 81-373) antibody (Biotin) (ABIN1169420)

Target: Caspase 4/11 p20

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunoprecipitation (IP), Western Blotting (WB)

Clone: Flamy-1

Product Details

Binding Specificity: AA 81-373

Specificity: Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11.

Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse Caspase-11 (aa 81-373).

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details

Target: Caspase 4/11 p20

Background: Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli. Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.

UniProt: P70343