IL-8 antibody (AA 23-101) (Biotin)

Catalog No. ABIN1172239

This Rabbit Polyclonal antibody specifically detects IL-8 in WB, IHC and ELISA. It exhibits reactivity toward Sheep.

Quick Overview for IL-8 antibody (AA 23-101) (Biotin) (ABIN1172239)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IL-8 Antibody (Biotin)

Binding Specificity: AA 23-101

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVLSRMST ELRCQCIKTH STPFHPKFIK ELRVIESGPH CENSEIIVKL TNGKEVCLDP KEKWVQKVVQ AFLKRAEKQD P

Specificity: It has been selected for its ability to recognize IL8 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant IL8 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IL8 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant IL8 (Ala23~Pro101) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy