MMP13 antibody (AA 62-217)

Catalog No. ABIN1172288

The Rabbit Polyclonal anti-MMP13 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect MMP13 in samples from Rat. There is 1 publication available.

Quick Overview for MMP13 antibody (AA 62-217) (ABIN1172288)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-MMP13 Antibody

Binding Specificity: AA 62-217

Purpose: Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

Specificity: The antibody is a rabbit polyclonal antibody raised against MMP13. It has been selected for its ability to recognize MMP13 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA099Ra01Recombinant Matrix Metalloproteinase 13 (MMP13)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-MMP13 antibody (ABIN1172288)

Chu, Dai, Wang, Tian, Song, Xiao, Shao, Zhang, Zhang: "Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis." in: BMC musculoskeletal disorders, Vol. 18, Issue 1, pp. 78, (2017) (PubMed).

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP13

Background: CLG3, Collagenase 3