CA 19-9 antibody (AA 35-361) (Biotin)

Catalog No. ABIN1172465

The Rabbit Polyclonal anti-CA 19-9 antibody has been validated for IHC, ELISA and WB. It is suitable to detect CA 19-9 in samples from Human.

Quick Overview for CA 19-9 antibody (AA 35-361) (Biotin) (ABIN1172465)

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA 19-9 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Product Details anti-CA 19-9 Antibody (Biotin)

Binding Specificity: AA 35-361

Sequence: MGHHHHHHSG SEF-RVSRDD ATGSPRAPSG SSRQDTTPTR PTLLILLWTW PFHIPVALSR CSEMVPGTAD CHITADRKVY PQADTVIVHH WDIMSNPKSR LPPSPRPQGQ RWIWFNLEPP PNCQHLEALD RYFNLTMSYR SDSDIFTPYG WLEPWSGQPA HPPLNLSAKT ELVAWAVSNW KPDSARVRYY QSLQAHLKVD VYGRSHKPLP KGTMMETLSR YKFYLAFENS LHPDYITEKL WRNALEAWAV PVVLGPSRSN YERFLPPDAF IHVDDFQSPK DLARYLQELD KDHARYLSYF RWRETLRPRS FSWALDFCKA CWKLQQESRY QTVRSIAAWF T

Specificity: It has been selected for its ability to recognize CA19-9 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant CA19-9 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CA19-9 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant CA19-9 (Arg35~Thr361) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for CA 19-9

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Alternative Name: Carbohydrate Antigen 19-9 (CA19-9)