CA 19-9 antibody

Catalog No. ABIN1042585

Product Details anti-CA 19-9 Antibody

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA 19-9 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Purification: IgM specific affinity chromatography

Immunogen: Ten precipitin lines obtained after immunodiffusion using monoclonal antibody NS 19-9 and mucins isolated from an ovarian cyst of a OLe (a+b-) patient

Clone: 121SLE

Isotype: IgM

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try at 2 μg/mL on FFPE tissue

Comment:

Myeloma, fusion partners: Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 myeloma line

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody in phosphate buffered saline containing 10 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-CA 19-9 antibody (ABIN1042585)

Partyka, Maupin, Brand, Haab: "Diverse monoclonal antibodies against the CA 19-9 antigen show variation in binding specificity with consequences for clinical interpretation." in: Proteomics, Vol. 12, Issue 13, pp. 2212-20, (2012) (PubMed).

Schnegelsberg, Schumacher, Valentiner: "Lectin histochemistry of metastasizing and non-metastasizing breast and colon cancer cells." in: Anticancer research, Vol. 31, Issue 5, pp. 1589-97, (2011) (PubMed).

Shi, Gao, Wang, Wang, Tian, Zhu, Shen, Qin: "CD133(+) gallbladder carcinoma cells exhibit self-renewal ability and tumorigenicity." in: World journal of gastroenterology : WJG, Vol. 17, Issue 24, pp. 2965-71, (2011) (PubMed).

Köhler, Ullrich, Richter, Schumacher: "E-/P-selectins and colon carcinoma metastasis: first in vivo evidence for their crucial role in a clinically relevant model of spontaneous metastasis formation in the lung." in: British journal of cancer, Vol. 102, Issue 3, pp. 602-9, (2010) (PubMed).

Rye, Bovin, Vlasova, Molodyk, Baryshnikov, Kreutz, Garinther, Schultes, Noujaim, Madiyalakan, Magnani, Nilsson, Nilsson, Nustad, Norum, Bell, Cao, Suresh, Very, Freeman, Yeung, Hilgers: "Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997." in: Tumour biology, Vol. 19, Issue 5, pp. 390-420, (1998) (PubMed).

Bara, Mollicone, Herrero-Zabaleta, Gautier, Daher, Oriol: "Ectopic expression of the Y (Ley) antigen defined by monoclonal antibody 12-4LE in distal colonic adenocarcinomas." in: International journal of cancer. Journal international du cancer, Vol. 41, Issue 5, pp. 683-9, (1988) (PubMed).

Target Details for CA 19-9

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Alternative Name: CA19-9    (CA 19-9 Products)

Background: Synonyms:
Sialyl Lewis (a) antibody,Sialyl Lewis(a) antibody,Sialyl Lewisa antibody