KRT16 antibody (AA 117-419)

Catalog No. ABIN1172993

The Rabbit Polyclonal anti-KRT16 antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect KRT16 in samples from Human. There is 1 publication available.

Quick Overview for KRT16 antibody (AA 117-419) (ABIN1172993)

Target: KRT16 (Keratin 16 (KRT16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-KRT16 Antibody

Binding Specificity: AA 117-419

Purpose: Polyclonal Antibody to Cytokeratin 16 (CK16)

Specificity: The antibody is a rabbit polyclonal antibody raised against CK16. It has been selected for its ability to recognize CK16 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA516Hu01Recombinant Cytokeratin 16 (CK16)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-KRT16 antibody (ABIN1172993)

Jørgensen, Lazzarini, Pirone, Jacobsen, Miragliotta: "Normal microscopic anatomy of equine body and limb skin: A morphological and immunohistochemical study." in: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, Vol. 218, pp. 205-212, (2018) (PubMed).

Target Details for KRT16

Target: KRT16 (Keratin 16 (KRT16))

Alternative Name: CK16

Background: KRT16, NEPPK, Keratin 16, Focal Non-Epidermolytic Palmoplantar Keratoderma, Keratin, type I cytoskeletal 16